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ELISA Tests For Gluten May Not Be Accurate

Posted by anna on April 13, 2022 

A new study conducted by RIKILT-Wageningen University of Technology in the Netherlands suggests that ELISA tests for gluten may not be accurate in all cases. While the current method is recommended by Codex Alimentarius, some tests have limitations. For instance, competitive R5 ELISA is not sensitive enough to detect the presence of gluten in cereals or fermented foods. Nevertheless, a type I method can detect gluten to parts per million.

A sandwich ELISA requires two epitopes on the gluten protein. Hydrolyzed proteins lack these epitopes, making them useless for the sandwich R5 ELISA. As a result, gluten-free foods, malt beverages, and soy sauce, may test negative. Although this doesn't mean that the product contains no gluten protein, it does suggest that the assay used is ineffective.

The sandwich R5 ELISA method uses a specific antibody called R5 to detect gluten. The sandwich ELISA has been the standard method for gluten testing in the food industry for over 30 years. However, it has limitations, especially when attempting to identify hydrolyzed fragments. Competitive R5 ELISA is more sensitive to smaller segments of protein in fermented foods, but it hasn't been fully validated.

Because gluten is a complex mixture of several hundred different proteins from three different species, ELISAs are not very accurate. This is due to the fact that ELISA tests are limited to detecting one type of protein and convert the results into the total gluten content. Most of the antibodies detect the prolamin fraction of gluten, which is the portion of the protein that binds to aqueous alcohol. Because gluten is so complicated, it's necessary to have a high-quality ELISA test. There maybe some residual substances on the ELISA plate after the detetion. In order to reduce the errors caused by the residues, an ELISA washer is needed. Elisa washer is a medical device specially designed to clean the microplate and generally used in conjunction with the microplate reader.

As a result, researchers are calling for urgent improvements to these testing kits. They recommend competitive formats, improved extraction methods, and detection of relevant gluten peptides. Nevertheless, it is important to note that if you test positive for gluten, you should consult a doctor. If your results are negative, you should continue eating your regular diet. If you have any family members who have positive results, it is vital to have early screening as a preventative measure.

To obtain results faster, RIDASCREEN Gliadin competitive ELISA offers a quantitative solution for gluten detection in beer. The MultiWash+ washer and SpectraMax ABS Plus reader automate the wash steps, while SoftMax Pro Software calculates results and flags results for further study. The RIDASCREEN Gliadin competitive ELISA is highly precise and sensitive and only takes an hour to reliably measure gluten content in beer.

The G12 antibody-based gluten detection kit was developed and evaluated for use in official control systems. Both tests were validated by comparing routine samples to samples that entered an official laboratory. They were compared against alpha 2-gliadin 33 mer from wheat and omega-Secalin from rye. This comparison demonstrates that G12 antibody-based ELISA is equivalent to the official R5 method.

The EZ Gluten(tm) Test can detect gluten in a wide range of food and beverage products, including beverages. It detects the presence of gluten as low as 10 ppm, and is portable enough for use in industry settings. This test also has the flexibility to detect individual ingredients in foods. So, if you are worried about gluten in food, EZ Gluten(tm) Test may be right for you.

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